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Abstract Creatine is a naturally occurring substance in the human body. It is synthesized from the amino acids arginine, glycine, and methionine, and is naturally produced by the liver, kidneys, and pancreas. The purpose of the study was to find out the effect of creatine monohydrate consumption on muscular power of south Gujarat male state level athletes. The study was delimited to the young male students of state level competitions and tournaments of various sports from South Gujarat region. The study was delimited to the young male students of state level competitions and tournaments of various sports from South Gujarat region. The period of training for the experiment group was eight weeks. There would be positive improvement in increase peak power, relative peak power (RPP), mean power, and relative mean power (RMP) after Supplementation of creatine monohydrate. Supplementation of creatine monohydrate will significantly lower fatigue index. To achieve purpose of the study Seventy Five (75) male participants in the age group of 18-25 years were randomly selected from South Gujarat region of the state level tournaments and competition. All the 75 male participants belonged to different colleges of Veer Narmad South Gujarat University. The study was formulated as pre and post-test random group design in which seventy five subjects were divided into three equal groups. The experimental group I (n = 25 CM group) consumed 5g creatine monohydrate with glucose, Protein and Milk for eight weeks. Group II (n = 25 PM group) consumed Glucose, Protein and Milk. Group III (n = 25 PT group) had not consumed creatine monohydrate glucose, protein and milk at all. The experimental group I, II and Control group were given training for 8 consecutive weeks (6 days in a week). Every training session will be lasted for 40 to 60 minutes. This chapter presents the results of the study from the data analysis of the experimental study. The analyses were carried out through various statistical techniques such as the dependent t-test, the univariate analysis of covariance (ANCOVA), and the post hoc pair wise comparison using the LSD test analysis. The data were compiled and analysed using the Statistical Package for the Social Science (SPSS)for windows computer software.