Published Paper Details:

Introduction: Pseudomonas aeruginosa is a Gram-negative bacterium with resistance to many antibiotics, causing severe infections in immunocompromised individuals. Resistance patterns vary geographically and over time, with beta-lactams showing high resistance to penicillins and cephalosporins, carbapenems increasing resistance, aminoglycosides having variable susceptibility, fluoroquinolones being common, and polymyxins reserved for multidrug- resistant cases. Management involves prevention, culture, prompt antimicrobial therapy, and combination therapy. In our present study, we will study the antibiotic sensitivity pattern in P. aeruginosa. Aim & Objectives: The aim and objectives of present study is to isolate Pseudomonas aeruginosa among various samples and estimate its antibiotic sensitivity pattern. Material & Methods: The study analysed antibiotic sensitivity patterns in Pseudomonas aeruginosa from clinical samples, using 50 non-repetitive isolates from outpatient and inpatient departments. The modified Kirby-Bauer disc diffusion method was used for antimicrobial susceptibility testing in the Department of Microbiology, National Medical College, Birgunj, Nepal, between 1 st July 2022 and 30 th June 2023. Results: Pseudomonas aeruginosa was found to be most abundant in pus samples, followed by ear swabs, urine, sputum, E.T. secretion, and blood. Out of 50 isolates, the most sensitive were Polymyxin-B (98%), Colistin (98), Imipenem (89%), Cefeparazone/Salbactum (86%), Meropenem (84%), Pipercillin/Tazobactum (82%), ciprofloxacin (72%), and cefepime (38%). Conclusion: The study identifies a drug for Pseudomonas aeruginosa infection, focusing on species-level identification and resistance mechanisms, promoting judicious use of chemotherapeutic agents