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In this study, copper nanoparticles (Cu NPs) were synthesized using Murraya koenigii leaf extract as a natural solvent that was safe for the environment. Murraya koenigii leaf extract has been found to decrease copper ions without the need for a stabilizer or surfactant agent, making it an easy, economical, and environmentally friendly way to prepare Cu Nps. A color shift in the reaction mixture was noticed upon the combination of plant extract with the copper sulphate solution, indicating the creation of nanoparticles. Using UV-Vis spectra, FESEM images and XRD spectrum the particles were characterized. UV-vis data shows that Cu NPs generated using this green synthesis approach remain stable even after a month, confirming their stability. Metallic Cu nanoparticles are less expensive than conventional heterogeneous catalysts. The characterization results of biosynthesized Cu NPs show that the particles are crystalline and cubical in shape, with an average size of 11.3 nm, and are very stable. This study has the potential to have a significant impact in a few years on the industrial synthesis of metallic nanoparticles. Because of its outstanding qualities, we suspected that the use of green-produced Cu NPs will result in the biomedical applications. Cu NPs exhibit cytotoxicity and anticancer characteristics in numerous applications.