Published Paper Details:

ABSTRACT: The field of medicine and research can greatly benefit from the application of genetic engineering. The Cas-9 protein and clustered regularly interspaced short palindromic repeats (CRISPR) are linked to a groundbreaking method in genetic engineering that allows for precise gene editing. CRISPR/Cas9 is an RNA domain-containing endonuclease-based genome engineering tool that has showed potential in cancer rehabilitation owing to its high precision, accuracy, time-saving, and cost-effective tactics. It has been utilized to enhance the clinical condition of Huntington's disease (HD), a rare neurological ailment caused by a CAG trinucleotide increase in the Huntingtin gene. It is distinguished by the considerable penetration of a single mutation, and it has been used to repair CF-causing mutations, making them effective therapeutic agents for combating HIV and other infectious diseases. In recent years, RNA-editing technologies based on clustered regularly interspaced short palindromic repeats (CRISPR)-associated nucleases 9 (CAS9) have come to light as potential treatments for this insidious illness. In this article the problem statement is find out how crispr cas 9 technology is used for effective treatment of various human diseases. Diseases to be covered in this article are cancer, huntington's disease, cystic fibrosis, human immunodeficiency virus (hiv) and alzheimers disease.