Published Paper Details:

ABSTRACT A Simple, Precise, Accurate and Economical UV spectrophotometric method was developed and validated for estimation of Colchicine in bulk and pharmaceutical dosage form. The drug was highly soluble in distilled water, so it was selected as the solvent system. The detection wavelength of Colchicine was found to be 246 nm. The linearity of Colchicine was found to be in range of 2-20 ?g/ml with correlation coefficient value 0.995.The linear regression equation obtained by least square regression method, were y = 0.0176x-0.0112. The absorbance was found to increases linearly with increasing concentration of Colchicine. The correlation coefficient of Colchicine was found to be 0.995. The LOD and LOQ were found to be 2.94 ?g/ml and 8.92 ?g/ml respectively. Mean recovery of Colchicine was found to be in the range of 98.06% to 100.77% signifies the accuracy of method. The methods was also found to be precise as % RSD was less than 2. This method was validated as per ICH Q2 (R1) guidelines. The proposed methods were found to be accurate, specific and reproducible which can be effectively applied to pharmaceutical dosage form. Robustness obtained % RSD Was found to be 0.827 and % Purity obtained by assay was 98.60%. KEYWORDS: Colchicine , ICH Guidline , Distilled water , Method Development, Validation. ACKNOWLEDGEMENT: Authors are very much thankful to Miss. Shweta C. Pote Assistant Professor of MAHADEVRAO WANDRE INSTITUTE OF TECHNOLOGY TURKEWADI KOLHAPUR, MAHARASHTRA.