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Background: Premenstrual syndrome (PMS) encompasses a range of physical, psychological, emotional, and mood changes that typically occur following ovulation in women. Globally, approximately 47.8% of girls in the reproductive age group experience PMS, with the highest occurrence observed in developed nations.1 Aims and objectives: The aim of this study was to assess the level of knowledge regarding PMS among adolescent girls and also to determine the association of knowledge with selected demographic variables among adolescent girls at selected schools, Punjab, India. Materials and Methods: A quantitative approach employing a descriptive research design was utilized in this study. A sample of 180 adolescent girls was selected based on specific inclusion and exclusion criteria, employing a nonprobability purposive sampling technique from selected schools in Punjab. Data was collected using a structured knowledge questionnaire. The obtained data was then tabulated and analyzed according to the study's objectives using descriptive and inferential statistics. Results: Findings revealed that majority of the adolescent girls (71.11%) had poor and (17.78%) had average knowledge level regarding PMS. A smaller portion of 11.11% demonstrated good knowledge, while none of the participants achieved an excellent level of knowledge regarding PMS. Overall adolescent girls were having average mean knowledge score. There was significant association of level of knowledge of adolescent girls regarding PMS with age and family history of PMS as statistically analyzed by ?2 value (25.76, P<0.05) and (32.53, P<0.05) respectively at 0.05 level of significance among adolescent girls.