Published Paper Details:

Tulasi, also known as Holy Basil (Ocimum sanctum), is revered in Hindu culture and prized for its medicinal properties. It aids in respiratory health, digestion, and stress relief due to its antimicrobial and adaptogenic qualities. Tulasi is used in traditional medicine for treating asthma, indigestion, and anxiety. It's also valued for its role in promoting heart health and maintaining oral hygiene. Culturally significant, Tulasi is worshipped for its spiritual significance and widely used in cooking for its aromatic flavour. Tulasi cultivation faces challenges such as climate sensitivity, pest susceptibility, and the need for precise watering and soil management. These factors can affect growth, yield, and overall plant health, requiring careful attention and management practices for successful cultivation. Tissue culture of Tulasi offers rapid multiplication of disease-free plants, ensuring genetic purity and uniformity. It enables year-round cultivation regardless of climatic conditions, contributing to consistent supply and preservation of this culturally significant plant. Additionally, tissue culture techniques facilitate research and development for enhancing Tulasi's medicinal properties and agricultural traits. in this present study evaluate the tissue culture of Tulasi plant from stem nodes in Murashige and Skoog. According to this study stem node turn green colour with in 4 weeks of incubation and started to form callus. On fifth week ending a small leaves developments were observed in stem nodal callus area from explants. In this state, we haven't noticed any root formation emerging from the callus.