Published Paper Details:

Nanotechnology is an emerging field that focuses on manipulating matter at the atomic and molecular scale, typically within the size range of 1 to 100 nanometers in one dimension. This technology has gained attention due to the unique behaviour of materials when reduced to nanoscale dimensions. One significant application is the utilization of nanostructured materials, such as silver nanoparticles, for removing harmful substances from air and water. Silver nanoparticles are renowned for their ability to eliminate bacteria. They are now being incorporated into next-generation dressings to combat infections. These nanoparticles can also be integrated into materials and fabrics, rendering them sterile. Recent research has revealed that silver nanoparticles can be produced through a green synthesis method using leaf extracts from the Azadirachta indica (neem). The presence of flavonoids and terpenoids in the extract serves as both reducing and capping agents. This study involved treating an aqueous solution of silver nitrate (AgNO3) with neem leaf extract, resulting in the formation of crystalline silver nanoparticles. The confirmation of nanoparticle formation was achieved using UV-visible spectroscopy, which displayed a peak absorption at 440 nm. The silver nanoparticles displayed antimicrobial properties against both Gram-positive and Gram-negative bacteria. For instance, they formed clear zones around them, with a diameter of 4 mm for Bacillus subtilis and 3 mm for Escherichia coli when using pure silver nanoparticles. Additionally, the crude extract of silver nanoparticles produced a 2 mm clear zone for Escherichia coli. Antifungal activity of Silve nanoparticles against Capnodium spp showed 4 mm which indicated the inhibition of fungal growth.