Published Paper Details:

The utilization of residual wastes from banana for the production of higher titre of cellulase enzymes in submerged fermentation was evaluated. Streptomyces aureofaciens (MTCC 325) exhibited faster and higher growth in the basal salt medium with banana agro waste compared to Streptomyces albidoflavus. The maximum cellulase production (6.641 IU/ml) of S. aureofaciens using glucose as a sole carbon source in submerged fermentation was comparatively higher than S. albidoflavus. Similarly, while using yeast extract as sole nitrogen source, S. aureofaciens has showed maximum cellulase production of 5.74 IU/ml, which was comparatively higher than S. Albidoflvus (4.88IU/ml). SDS-PAGE analysis showed that the molecular weight of the partially purified enzyme was 70 kDa and 72 kDa for Streptomyces aureofaciens and Streptomyces albidoflavus respectively. The optimum culture conditions for cellulase production using the above two novel actinomycetes were also studied. The maximum cellulase activity was observed on the 5th day of incubation at 35-45?C, at the pH range of 6.0 - 7.0 for both the strains.