Published Paper Details:

Stemformatics is a newly introduced platform of bioinformatics which deals with stem cell related in-silico information and a well-known source of gene expression datasets that have been compiled using single cell profiling, RNA sequencing, and microarray technologies. It is also a well known data portal of stem cell gene expressions. It allows for simple to view and compare gene expression profiles in mouse and human across many platforms from various lab sources. Our group also has preliminary omics study of translation applications in CRISPR CAS 9 Gene by exploiting Stemformatics tool viz: phylogeny along with the transcriptional factors and CpG islands analysis. Using "molecular scissors (CRISPR)," genome editing involves adding, and replacing DNA in a living organism's genome. Traditional genome editing for human stem cells using designed nucleases has limitations by its ineffectiveness, Cost effective, and lack of selectivity. The CRISPR system has recently come into prominence as a potent gene-editing method with benefits of high editing efficacy. Despite the enormous potential for gene modification in a variety of species, from prokaryotes to higher mammals. The sample annotations are not adequately standardized, the abundance of primary data in stemformatics creates difficulties for data aggregation and downstream analysis. So, in this article, we focused on the underlying biology and use of the CRISPR/Cas9 system in current human stem cell research, in-silico evaluations of its advantages and future applications of human stem cells in regenerative medicine.