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Background: The rate of deceased donations in India is currently between 0.05 and 0.08 per million population. The National Organ and Tissue Transplant Programme has developed plans for raising awareness and developing capacity in order to enhance organ donation. There is a need that knowledge and attitudes regarding organ donation among the population be initiated to promoting awareness and increasing donation rates.1 Aim of the study: The study aimed to assess the effectiveness of a Structured Teaching Program (STP) on enhancing organ donation knowledge and attitudes among first-year B.Sc. Nursing students at at a selected college, Punjab, India Material and Methods: This quantitative study was conducted among B.Sc. Nursing (First Year) students selected by non-probability convenience sampling technique from a selected college, Punjab, India.The study employed a single group pre-test and post-test pre-experimental research design. Tools used were self-structured knowledge questionnaire and a five point Likert scale for attitude. Results: In the pretest, 54% of the sample had good knowledge, and 42% had average knowledge. After the structured teaching program, 98% gained excellent knowledge, with 2% maintaining good knowledge. Initially, 74% had a positive attitude, and 26% had a neutral attitude. Post-program, 26% shifted from neutral to positive attitude. The pretest overall mean score was 10.60 (SD 2.129), increasing in the post-test to 17.620 (SD 1.2103). Pretest attitude mean was 59.56 (SD 6.058), rising to 62.06 (SD 4.137) post-test, with a significant 't' value of 3.667 at P<0.05. The results indicate a substantial improvement in awareness and positivity post-education on organ donation.