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ABSTRACT Background: The prevalence of cardiovascular diseases is increasing in developing countries due to population growth, aging, unhealthy diets, obesity, and sedentary lifestyles. Over 30 million have now been diagnosed with coronary artery diseases in India. The CPR (Crude prevalence rate) in the urban areas of India is thought to be 13 percent. It is essential, to have knowledge regarding interpretation of electrocardiograph in myocardial infarction among the Staff Nurses. Methodology: A pre experimental method was undertaken where 100 Staff Nurses working in selected hospitals of metropolitan city were recruited as sample by non- probability convenient sampling method. Using structured questionnaire for knowledge (30 questions) and data were collected. Data analyzed using both descriptive and inferential statistics to describe and show the association between the variables. Results: Out of 100 Staff Nurses in pre- test knowledge scores, 15% of subjects were having good knowledge, 73% were having average knowledge and 12% in the poor knowledge category, Whereas In in post- test knowledge scores, out of 100 Staff Nurse 94% of subjects were having good knowledge, 6% were having average knowledge and no one in the poor knowledge category. Paired t' test was used to assess the effect on knowledge regarding ECG interpretation, the calculated t' value (17.29) as more than table value (1.98), thus the Null hypothesis (H0) is rejected and alternative hypothesis (H1) is accepted. Knowledge scores were found to be not associated with age, gender and working experience (P > 0.05). Knowledge score were found to be associated with education and working area (P <0.05). Here the other Null hypothesis (H01) is rejected and other alternative hypothesis (H2) is accepted in some specific demographic variables. Conclusion: The knowledge of Staff Nurses working selected hospitals was increased after giving the structured teaching program, thus the structured teaching program was found to be effective and It is recommended that frequent training programs on such topics has to be arranged for Staff Nurses in each hospital.