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ABSTRACT Background:- Diarrheal disease is a major cause of morbidity and mortality throughout the world, particularly in developing countries. Currently available drugs are linked with adverse effects, contraindications, and risk of resistance. Traditionally, the Dried leaves concoction of Kalanchoe Pinnata Lam. is claimed to be used for diarrhea. However, the safety and efficacy of the leaf extract have not been scientifically approved yet. Therefore, the study was conducted to validate its antidiarrheal activity and safety profile in mice. Method:- The extract was obtained by the maceration technique in 95% Ethanol and water. Phytochemical screening tests were done for secondary metabolites by using standard tests. The antidiarrheal activity of the test extract at the doses of 150 mg /kg and 250 mg /kg was evaluated by using castor oil-induced models in mice. Result:- In an acute toxicity study, there were no visible signs of toxicity and mortality following a single oral administration of 2000 mg/kg. Phytochemical screening tests revealed the presence of of Ethanol extract showed presence of Alkaloids, Proteins, Carbohydrate, Phenols and Tannins, Flavonoids, Saponins, Glycosides, Triterpenoids. And Aqueous extract showed presence of Alkaloids, Proteins, Carbohydrate, Phenols and Tannins, Flavonoids. Saponins, Glycosides, Triterpenoids. The extract significantly prolonged the onset of diarrhea and reduced the weight of wet and total feces at Group of mice treated with castor oil (0.3 ml) are called disease control group showed a significant (P<0.05) increase the biochemical parameters. Group of mice which pre- treated with Loperamide (2 mg/kg orally) was standard group, expresses significant *(P<0.05)* inhibition of increase the biochemical parameters group of mice which were pre- treated with Ethanolic Extract (150 and 250 mg/kg orally ) demonstrate significant *(P< 0.05)* effect of Ethanolic Extract on biochemical parameters. Conclusion:- The study demonstrated that the test extract showed promising antidiarrheal activity.