Published Paper Details:

Use of PCR techniques in the field of Microbiological, diagnostics and Forensic Science has increased to the point where it is now accepted as the standard method for detecting nucleic acids from a number of sample and microbial types.In the scientific laboratory, traditional PCR was already a necessary instrument, though. Due to its increased speed, sensitivity, and reproducibility as well as its reduced danger of carryover contamination, real-time PCR has facilitated the wider use of PCR. During real-time PCR, a growing variety of chemistries are employed to detect PCR products as they build within a closed reaction vessel. PCR is being used in two key areas in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also address the commercialization of these techniques before concluding with an examination of their modifications and application in novel areas of biomedicine.. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. The gold standard for SARS-CoV-2 diagnosis is real-time reverse transcription PCR. The dynamic range of initial target molecule determination in real-time PCR is very large (at least five orders of magnitude). Real-time quantitative PCR is more accurate and requires less labor than traditional quantitative PCR procedures.