Published Paper Details:

Cassia alata, commonly known as the ringworm bush or Senna alata, is a medicinal plant widely recognized for its therapeutic potential. This study aimed to perform a comprehensive phytochemical analysis of the leaf extract of Cassia alata using High-Performance Thin-Layer Chromatography (HPTLC) and identify the presence of bioactive compounds. The leaves of Cassia alata were collected, air-dried, powdered, and subjected to sequential extraction using appropriate solvents. The resulting extracts were concentrated, and the dried residue was dissolved in suitable solvents for HPTLC analysis. Silica gel-coated plates were used as the stationary phase, while different mobile phases were employed to achieve optimal separation and resolution of compounds. Preliminary phytochemical screening of the leaf extract revealed the presence of proteins, amino acids, sugars, monosachharides, alkaloids, tanins, sterols, saponins. HPTLC analysis provided detailed information about the phytochemical composition of the leaf extract by comparing the chromatographic profiles with standard compounds and applying suitable detection methods. The HPTLC analysis of Cassia alata leaf extract identified several bioactive compounds, including flavonoids such as quercetin, kaempferol, and their derivatives. These flavonoids are known for their antioxidant, anti-inflammatory, and antimicrobial properties. Additionally, other compounds, such as phenolic acids, alkaloids, and terpenes, were also detected in the leaf extract, suggesting their potential therapeutic activities.