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In this modern era, medicinal plants are important for the drug industries for the production of therapeutic compounds. Hence the present study was aimed at determining phytochemical analysis of leaves of locally grown Nyctanthes arbortristis, Murraya koenigii plants for their antibacterial activity against selected bacterial pathogens. For the present study, herbal extracts of leaves of Nyctanthes arbortristis and Murraya koenigii were prepared by Cold maceration method by using methanol as solvent. The antibacterial activity was determined by the agar well diffusion technique and the prepared extract was evaluated for physicochemical characters. The bioactive compounds present in the methanol extract a fraction of Nyctanthes arbortristis and Murraya koenigii leaf were identified by GCMS analysis. The maximum antibacterial activity was observed for a methanolic extract of Nyctanthes arbortristis than Murraya koenigii. Both the methanolic extracts exhibited maximum activity against the organism Staphylococcus aureus. The physicochemical characters showed the presence of carbohydrates, alkaloids, tannins, cardiac glycosides, fatty acids, and oils. The GCMS analysis showed the presence of 45 peaks indicating the presence of 45 compounds in both the methanolic extract of Nyctanthes arbortristis and Murraya koenigii. The Constituents obtained from Nyctanthes arbortristis extraction with high quantity were 2-(4-Methoxyphenyl)ethanol(10.46), Neophytadiene(10.33), n-Hexadecanoic acid(8.95) and in Murraya koenigii extraction 1-Methyl-pyrrolidine-2-carboxylic acid(41.80), Phytol(14.66), and Caryophyllene(6.50) were present in high amounts. It can be concluded that leaves of Nyctanthes arbortristis and Murraya koenigii can be used for therapeutic purposes.