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Donkey milk is the milk from the domesticated donkey. It has been used since antiquity for cosmetic purposes as well as infant nutrition.Donkey milk has been used by humans for alimentary and cosmetic purposes since Egyptian antiquity. Doctors recommended it to treat several afflictions, due to its healing and cosmetic virtues. Donkey milk (DM) has gained scientific attention in recent years.Because it has compelling nutrients and functional elements.(Mansueto et al. 2013 There are much similarites in chemical compositions of human milk and donkey milk.Donkey's milk is used as an alternative for infants with multiple food allergies and intolerances.Recently, fermented beverages based on donkey milk were proposed as important sources of probiotics and antioxidants with several health benefits. Donkey milk contains high levels of lactose and minimal amounts of casein and fat. One of the main particularities of Donkey milk is its high lysozyme's content. Lysozyme shows antibacterial activity against numerous Gram-positive bacteria; including lactic acid bacteria (LAB). Recently, overuse of antibiotics has led to the emergence and spread of high bacterial resistance, making difficult to treat infections involving tremendous research efforts by the medical and scientific community(Chiavari et al. 2005).In fact, living organisms have developed an immense molecular diversity, containing ubiquitous low molecular weight secondary metabolites isolated from plants, insects, marine organisms, and other microorganisms, including lactic acid bacteria (LAB).