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ABSTRACT BACKGROUND: A meta- analysis of the studies available through the Cochrane database and WHO's reproductive health library confirmed that active management of labour was associated with reduced PPH, anaemia and decreased need for Blood Transfusion ,reduced risk of prolonged third stage and less use of addition a uterotonic drugs. Midwives are skilled health care professionals. At this point, it was strongly felt that structured Teaching program will illuminate their minds and improve their knowledge and practice and ultimately to update the current knowledge and practice in this field. METHODS: The study design consisted of one group pre-test and post-test pre experimental research design. Non -probability purposive sampling technique was used for selection of samples. The data was collected from 50 staff nurses working in maternity department of HI-TECH Medical College and Hospital, Bhubaneswar, Khurdha, Odisha using Close ended questionnaire to assess the knowledge and Observational checklist to assess the practices for performing active management of third stage of labor (AMTSL). Post test was conducted after 7 days of administration of STP to know the effectiveness by using the same close ended questionnaire and observational checklist. RESULTS: In pre-test; nurses are having on an average 49.04% of knowledge scores on AMTSL. In post-test after implementation of STP, the staff nurses scored up to 76.25% .In pre-test the practice scores is 39.73% .In post-test, after implementation of STP the nurses have scored 63.47%. The difference of knowledge scores between pre-test and post-test is 10.74 and the difference of practice scores between pre-test and post-test is 5.46.Thepaired't' test was calculated and found that there is a significant difference between pre and post-test knowledge (6.71) and practice scores (5.80) before and after implementation of STP. Karl Pearson's coefficient correlation between knowledge with practice of pre-test(r = .70) and post-test (r=0.76) shows significant relationship, which reveals that when knowledge level increases the practice will also increases. There is association between post-test knowledge and sex of staff nurses and practice is the significant association between areas of working, of staff nurses is calculated by using Chi -square test at 0.05 level of significance. CONCLUSION: The result of the study shows that; there is a great need for the staff nurses, to update their knowledge and practices regarding AMTSL.