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Herbal medicine also known as herbalism or phytotherapy is a type of alternative medicine that uses plant extract, herbal, and other natural substances to prevent and treat various health conditions. It is one of the oldest forms of medicine and has been used by different cultures around the word for thousands of year. The ultimate aim of this study is to formulate and evaluate the herbal lotion using herbal extracts of plants having ethnic and dermatological importance in Ayurveda, namely, Leucas aspera, and Ocimum tenifloram. In the current scenario in cosmetics, the use of herbal antifungal lotion containing natural ingredient are more acceptable in public belief. In the formulation we utilizes Tulsi leaves extract, Aloe Vera gel, Rose water, and Leucas aspera leaves extract which are not yet used by any other research work. The plant leaves contain active ingredients that have pharmacological effect on the body and can be used for different purposes. It have possess the various pharmacological activities like antifungal, antioxidant, antitoxic, and antimicrobial activity.