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In the present study, Exploratory Research Design was adopted for the present study was conducted in a Civil hospital in Fatehgarh Sahib, Punjab. The population in the present study included 60 OPD Patients present at the time of data collection, the sample was drawn by Non-Probable Convenient sampling technique. The data were collected by a structured demographic sheet and self-structured questionnaire. The frequency and percentage of the level of knowledge among OPD patients about self-insulin administration depict that most (55%) of OPD patients had good knowledge followed by (43.66%) and few (1%). Thus, it is concluded that diabetic patients had good knowledge regarding self-administration of insulin. Frequency and percentage distribution level of practice regarding self-administration of insulin among the OPD patients depicts that (100%) OPD patients had desirable practice regarding self-administration of insulin. Hence, it was inferred that OPD patients had practice regarding self-administration of insulin. The Co- relationship between the knowledge and practice regarding self-administration of insulin among OPD patients of civil hospitals shows a relationship value between the knowledge score and practice score of 9.581. It depicts that there was a positive relationship between knowledge and practice regarding self-administration of insulin among OPD patients. Thus, it was concluded that knowledge of OPD patients increases regarding self-administration of insulin among OPD patients. There is no association between knowledge and practice concerning selected variables such as age, gender, qualification, duration of insulin, type of injection, and source of information. It was concluded that the majority of the adults know i.e.: 55% regarding self-administration of insulin.