Published Paper Details:

Micropropagation of Ashwagandha was done via the process of Plant Shoot tip Tissue Culture techniques where the explants were taken as shoot tips from stock plants of ashwagandha, and growth hormone was taken as BAP, in nutrient media of Murashige and Skoog. Five nutrient medias were prepared using five different hormone concentrations of BAP (2mg/L, 2.5mg/L, 3mg/L, 3.5mg/L, 4mg/L). Ten shoot tips were taken from stock plants and surface sterilised for inoculation in the media jars (2 explants per jar). After inoculation, the inoculated explants were incubated in the incubation room in photoperiod racks. The room temperature was maintained at 24-28 degrees celsius, where 17 hours of daytime and 7 hours of night time were provided to them. The explants were kept in incubation for 25 days, and it was observed that the explants which were inoculated in the media with hormone concentration of 3mg/L, responded the best, providing a total of 10 branches and final length of 4.7 centimetres, from the initial stage of having 3 branches and 2.1 centimetres in length.