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ABSTRACT:- The main aim to present work is to formulate and evaluate poly-herbal hand wash byusingaloe-vera,lemon juice.Inordertomakeformulation haslesssideeffectsandbettercleaningofhands.Thehandsaraprimarysitesfortheinfection.MicrobialinfectioniscriticalIssueinchildrenandemloyerinpharmaceuticalindustry.SotheuseofhandwashismoreinindustrialsiteTheformulatedhandwashhacefoundtobe good in physical parameter with good cleaning of hands.Therefore it brings up theuse of antiseptic for hand washing purpose.The prepared handwash evaluated by thediffrentparameternamelyascolour,odour,ph,viscosityandstability. Handsareprimarymodeoftransmissionofmicrobesandinfections.Hand-washingis critical in food production, food service and also important in healthcare setting,homes and day care preparations. The present research was aimed to evaluate theantibacterial efficacy of various herbal oils such as Cinnamon oil, Eucalyptus oil,menthol oil and lavender oil and found that cinnamon oil showed better antibacterialactivity. Also the research was carried out to formulate and evaluate the poly herbalHandwashgel containing Cinnamonoil. The anti-microbial activity of the formulated herbal hand wash gel was tested againstEscherichiacoli, Staphylococcus aureus and Salmonellaby spread plate techniquesand the results obtained were compared with commercial antibacterial standards. Alsothe efficiency was checked by using the hand wash gel on volunteers. The results fromthe present work suggest and support the incorporation and utilization of herbs in theformulationstogivebettereffect. Herbalmedicinesaresignificantpartofhealthcarethroughouttheworld.Herbalmedicines have been extensively utilized as effectual remedies for the prevention andmanagementof multiple health conditions.Hands are a prime mode oftransmissionofmicrobesandnosocomialinfections.Handwashingisextremelyimperativeinhealthcare and domestic sector. Numerous of the antiseptic hand wash available in themarket are alcohol based sanitizers which have some adverse effects. To avoid theseadverseeffectslikeitching,drying,irritation,dermatitisetc.,ofthesynthetichandwash formulations an attempt has been made to formulate a herbal hand washusing extracts ofEucalyptus globulus extracts. Theresults from the present worksupport the incorporation and utilization of herbs in the formulations to give a bettereffect. Herbal hand wash evaluated by tested parameters like physical parameters like colour,fragrance and chemical parameters like pH, Viscosity, Foam height, Foam retention,Skinirritationtestetc.andobtainedresultswereintheacceptablelimitswithlessornosideeffects.