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ABSTRACT �Nosocomial� or �healthcare associated infections� (HCAI) appear in a patient under medical care in the hospital or other health care facility which was absent at the time of admission. These infections can occur during healthcare delivery for other diseases and even after the discharge of the patients. Additionally, they comprise occupational infections among the medical staff. Invasive devices such as catheters and ventilators employed in modern health care are associated to these infections. Populations at stake are patients in Intensive Care Units (ICUs), burn units, undergoing organ transplant and neonates. In pediatric unit Hospital Acquired Infection presents a different set of challenges than adult general ward Hospital Acquired Infection measures consider being successful. The present �A study to assess the effectiveness of informational booklet regarding Hospital Acquired Infection and its prevention among the parents of under five year children in pediatric unit of SMIH Patel Nagar Dehradun� AIM: To assess the effectiveness of informational booklet on knowledge regarding Hospital Acquired Infection among patients of under five year children at SMIH, Patel Nagar Dehradun OBJECTIVE OF THE STUDY 1. To assess the knowledge of hospital acquired infection among parents of under five 2. To assess the effectiveness of information booklet program on hospital acquired infection through knowledge score. 3. To find the difference between pre-test and post-test knowledge score. METHODOLOGY The nature of the study was pre-experimental. The study was conducted in SMIH, Patel Nagar, Dehradun, the research design use for the study was one group pre-test and post- test design. Data collected by using structured questionnaire. The data collected for to assess the effectiveness of information booklet on knowledge regarding hospital acquired infection among parents under five children. RESULT According to the score between 0-9 had inadequate knowledge (78%) and 10-17 had moderate knowledge (22%) about Hospital Acquired Infection. CONCLUSION: It can concluded that pre- test knowledge score was less amount in parents. After implementing informational booklet the knowledge the knowledge score was increased. So it was affected in increasing knowledge.