Published Paper Details:

The aim of the current study was to evaluate the antimicrobial activity of the Eugenia Caryophyllus oil and Trigonella foenum-graceum L. The aqueous flower bud extracts of Eugenia Caryophyllus and aqueous seed extract of Trigonella foenum-graceum prepared and tested qualitatively, by screening of phytoconstituents that present in the extracts and to quantitatively by checking antimicrobial activity using well diffusion agar plate method. Agar cup plate test was used to determine the sensitivity of the tested samples while the well microdilution was used to determine the minimum inhibitory concentration. The drug extract were extracted using solvent extraction by soxhlet apparatus, different concentration of these extract were prepared ranging from 0.1-1.0 ml and antimicrobial activity is performed on Staphylococcus aureus (gram positive) bacteria, using nutrient agar as broth. It was found that the above drug combination Eugenia Caryophyllus & Trigonella foenum-graceum on the respective gram positive bacteria Staphylococcus aureus was showing optimum antimicrobial activity.The minimum inhibitory concentration of combination of both drugs was found to be 8.0 mm for the concentration of 0.9 ml and 1.0 ml for both drugs Clove oil & Fenugreek seed Extract. Chewing gum preparation shows antimicrobial activity in the range of 4.8mm to 5.5mm. The overall results of this study indicate that the chewing gum prepared from both the drug extracts have optimum antimicrobial and potential for treating Halitosis.