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ABSTRACT A nosocomial infection is also known as a hospital-acquired infection,(HAI) is an infection which is developed by the hospital environment, such as one acquired by a patient during a hospital visit or one developing among hospital staff. Such infections include fungal and bacterial infections and are aggravated by the reduced resistance of individual patient. Urinary tract infections are responsible for over a third of all hospital acquired infections. Most of these (at least 80%) follow some type of invasive procedures or instrumentation of the urinary tract, usually catheterization. Urinary tract infections associated with urinary catheters is the leading cause of secondary nosocomial bacteremia. Approximately 20% of hospital- acquired bacteremia are due to indwelling catheters and the mortality associated with this condition is about 10%. At this point it was important to prevent the occurrence of catheter associated urinary tract infection through a proper catheter care and to promulgate the best solution to be used for catheter care among patients with indwelling catheter in preventing catheter associated urinary tract infections. Methods: The research design used in the study was randomized block design. Non-probability purposive sampling technique was used for selection of samples. The data was collected from 30 catheterized patients using CAUTI checklist. Post test was conducted after 5 days of catheter care to know the incident rate of Urinary Tract Infection using the same CAUTI checklist and urine microscopy reports. Results: The overall mean percentage in the pre test and post test was, In Betadine group (Experimental group A) the pre test mean percentage is 37.39% and the post test mean percentage is 75.65%. Enhancement in mean percentage is 38.26%. with the significant t value 6.24 at 0.05 level. In Normal saline group (Experimental group B) the pre test mean percentage is 37.39% and the post test mean percentage is 76.15% the Enhancement in mean percentage is 38.76% with the significant t value 6.69 at 0.05 level. There is no significant association found between the incident rates of Urinary Tract Infection and the selected demographic data. The study findings shows that normal saline is more effective when compared with betadine in preventing the occurrence of urinary tract infection among catheterised patients.