Published Paper Details:

The in vitro cultivation of mature plant shoot tip explants was used to construct an in vitro micropropagation strategy for the annual medicinally Gloriosa superba L. Shoot tips from 30-35-day-old field-grown plants were employed for in vitro micropropagation shoot tip explants culture. Surface sterilizing of shoot tips was found to be most effective after three minutes in 0.1% HgCl2 solution. For the main establishment of isolated shoot tips on MS medium supplemented with (BAP/Kn/TDZ) (0.5-3.0mg/L), IAA (0.5 mg/L) + (BAP /Kn/ TDZ) (0.5-3.0 mg/L), and (BAP/Kn/TDZ) (0.5-3.0 mg/L) for multiple shoot induction. Within four weeks of culture, multiple shoot bud proliferation was seen at IAA (0.5 mg/L) + (2.0 mg/L) TDZ from shoot explants. The number of shoots per explant varied between 2 and 6. Several shoots were under aseptic conditions removed and subcultures in the same medium to extend the shoot. For root induction, the possibly resulting were transplanted to IAA/IBA (0.0mg/L-2.0mg/L). Within two weeks of culture, rooting was seen. Rooted plantlets were successfully toughened under culture conditions before being planted in the field. The recorded plant survival percentage was 86%. The plants appeared to be in good condition, with no visible phenotypic differences. With an 87% survival rate, the plantlets (12-16 weeks old) were successfully acclimatized to soil.