Published Paper Details:

Finger millet (Eleusine coracana L. Gaertn) belongs to the Poaceae family and is a staple crop in Africa and India. It is more resilient to stress than other crops and provides nutrients like calcium, zinc, and protein. Here We required DNA isolation for molecular characterization by Scot (Started Codon Targeted) markers. Thus, the CTAB method was best for DNA isolation. So we determined a rapid and easy CTAB (Cetyl trimethyl ammonium bromide) method for isolation of gDNA from finger millet based on four modified protocols due to the crop's containing polyphenol and PCR inhibitors. The first protocol of the CTAB method was superior to another modified CTAB method. Another hand We optimised the PCR amplification for Scot. The result was monomorphic according to band position. Furthermore, we developed a protocol among four protocols for the isolation of gDNA from finger millet where it was a convenient and easy method for extracting DNA from finger millet, also minimising the number of steps and chemicals. The first protocol was superior to the remaining protocols as an easy and convenient method for DNA extraction in finger millet. As well as being beneficial for DNA isolation, this procedure will provide new insight into research. We also established a protocol using the CTAB method, which is an easy and convenient method for DNA extraction in finger millet.