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ABSTRACT Nowadays, cosmetics are in high demand in everyday life, and many people use them on a regular basis. Herbal cosmetics are popular because they have fewer negative effects and provide higher protection and safety. People who are experiencing hair loss are looking for ways to increase hair development and prevent hair loss. Scalp serums moisturise the skin behind the hair, making them a popular treatment for dry scalp. The formulation mainly contain concentrated ingredients such as nettle leaf, bhringraj, and amla. Urtica diocia (nettle leaf extract) can aid to preserve our scalp from damage while also stimulating new hair growth. Hair loss, split ends, dandruff, balding, and premature greying are all treated by Eclipta alba (Bhringraj). One of the most important medicinal plants, Phyllanthus Emblica Linn (Amla), offers necessary minerals and the highest level of vitamin C. It also promotes hair growth, pigmentation, and the prevention of greying. Vitamin E, Aloe vera, Biotin, Guar gum, D-panthenol, and essential oils of Rosemary, lavender, and clarysage were added to the aqueous extract of nettle leaf, bhringhraj, and amla, to improve the effect in the scalp. The prepared medicated scalp serum was evaluated, and several characteristics such as physical appearance, viscosity, spreadability, and antimicrobial contamination were determined and documented. The colour of the prepared scalp serum was discovered to be brown. The formulation had a smooth and lubricating texture, and the pH was within the permissible range. The formulations were found to be stable when placed in the stability chamber and also found to have good antibacterial activity.