Published Paper Details:

The article reviews the different in-vitro assays can be carry out in the laboratory to check the anti-inflammatory activity for particular herbal extracts. These assays include protein denaturation assay, membrane stabilization assay, enzyme assays. Diclofenac, acetyl salicylic acid, indomethacin used as standard drugs in the following assays. all assays are possible to carry out inside the laboratory, so there is no animal involved and harmed and herbal drug shows less side effects than synthetic one with easy availability. The anti-inflammatory activity can be check for herbal drugs in less time, in-vitro by following methods. Protein denaturation assay includes heating of egg albumin it denaturate the protein and shows the anti-inflammatory activity.membrane lysis assay involves preparation of red blood cells suspension, then the heating of solution, centrifugation, and then absorbance is taken of the supernatant layer.hypotonicity induced haemolysis include hyposaline for anti-inflammatory activity. enzyme assays include collection of enzyme from animal cells or tissues. Enzymes such as lipoxygenase, cyccloxygenase, hyaluronidase from animal tissues or cells.activities of enzymes takes place through reactions carried out under certain conditions are used for in-vitro study not the animals. Antiproteinase activity involves incubation, perchloric acid which terminate the reaction,centrifugation and absorbance.in lipoxygenase enzyme assay linoleic acid used as substrate to start the reaction, absorbance and calculation gives inhibition of lipoxygenase by using equations.