Published Paper Details:

Euphorbiaceae is that the family of Euphorbia hirta plant. It's distributed throughout the place ladmass, they found in waste places on the roadsides. The plant components area unit wide utilized in ancient system of medicines, within the treatment of metabolism diseases, epithelial duct disorders, wound healing, respiratory organ disorders, urinogenital disorders, tumors, lactation in ladies etc. The current study, ethanolic extract of genus Euphorbia hirta has been aimed to analyse the phytochemical analysis, TLC (Thin Layer Chromatography), MTT ASSAY, acridine orange-ethidium bromide fluorescent staining methodology. The results analysis for phytochemical analysis might include the presence of terpenoids, saponins, steroids, alkaloids, tannins, flavonoids, betacyanin, comarins respectively. For TLC steroids, alkaloids and terpenoids where present in spot look for Rf value was calculated 0.90, 0.88, 0.95 respectively. MTT results shows the IC50 value of EH sample is 112.20% and also the 5-FU sample become 2.79% respectively. Then the AO/EtBr dual staining the results might include the red color become an dead cells, the yellow color become an ethanolic extract of EH treated sample, and also the green color become an viable cells. When these are the results might analysed against the Human Colorectal Adenocarcinoma were investigated using HT29 Cell line. For the future studies can development of anticancer drugs from medicinal plants EH.