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This report is based onmy work in the Central Laboratory of Sharda Hospital. The main discussion here is about techniques and procedures that are usually opted for diagnosis and identification of pathogens and disease causing agents, which can be useful for the purpose of research also. Broadly divided into Biochemistry, Microbiology (Bacteriology, Serology ,Virology and Mycology) and Pathology. In Biochemistry techniques such as Chromatography and its types including - Thin-layer chromatography (TLC), Column chromatography and High-performance liquid chromatography (HPLC) as well as basics of Spectroscopy UV-Visible spectroscopy, Infrared spectroscopy (IR) and Fluorescence spectroscopy. Mass Spectrometry and Enzyme Assays along with Protein purification techniques such as Affinity chromatography, Size-exclusion chromatography , NMR (Nuclear Magnetic Resonance) spectroscopy and Bioinformatics. In Microbiology methods and techniques in Bacteriology mainly focused on culturing Bacteria and processing them through different staining methods, biochemical testing and smear preparation. Serology Lab mainly use strip testing (The kit contains indicators to visualize the reaction between antibodies and reagents. This could involve color changes, fluorescence, or other methods depending on the design of the kit). Virology and Molecular Laboratory employs techniques such as ELISA, RT-PCR and methods for DNA extraction. Working in Pathology Lab teaches us about Histopathology(This involves the microscopic examination of tissue samples to study the cellular structure and identify any abnormalities. Tissues are typically stained with dyes to enhance visualization), Cytology, Immunohistochemistry (IHC) and Clinical Chemistry, techniques include spectrophotometry, chromatography, and immunoassays. Along with Flow Cytometry used for Hematological analysis to evaluate blood samples to assess the cellular components (red blood cells, white blood cells, and platelets) and their characteristics. Other techniques include complete blood count (CBC), blood smear examination, and coagulation assays.