Published Paper Details:

The main objective is development and characterization of ciprofloxacin microsphres as sustained release dosage form. Preformulation study was carried out initially and Drug (Ciprofloxacin) was identified by different methods. UV spectrophotometric study, FTIR spectra and was performed for the authentication of the drug. The ?max was found to be 276.0 nm and comparison with literature value authenticates the study. Calibration curve of the drug was prepared. The absorbance was measured at 276.0 nm against a blank solution using UV-Visible spectrophotometer. Linearity was observed with an R2 = 0.998 in 0.1N HCL at 276.0 nm.The drug-Exciepients compatibility studies using FT-IR and indicated no interaction between the drug and polymers. The microspheres prepared by two different technique for using synthetic polymer and natural polymer utilizing temperature change showed a good specificity, with smooth surface and the particles are distributed uniformly without any lumps. In synthetic polymer (Ethyl cellulose) prepared by solvent evaporation technique and natural polymer (guar gum) ionic cross linking technique. Scanning electron microscopy for the optimized formulation for natural and synthetic shown in fig. 7.8 and 7.7. The particle size is formulated batches of microspheres were shown in table No.7.9. The particle size distribution was uniform and narrow. The release profile of microspheres was studied for first two hours in stimulated gastric pH using 0.1N hydrochloric acid. The percent of drug release was calculated by adding the amount of drug released at the end of 2nd hour in stimulated gastric pH to the amount of drug released in stimulated intestinal pH. The whole release study data was given (Table: 7.10 and 7.11). As the compare of EC and Guar gum release the best sustained effect over a period of 24 hours and ethyl cellulose microsphere was 97.913 % of the drug was released at the end of 24 hours.