Published Paper Details:

A significant proportion of nosocomial and many infections are contributed by the Pseudomonas spp. major changes were observed from time to time in Pseudomonas sp. prevalence and multidrug-resistant (MDR) with enormous morbidity and mortality. The spread of antimicrobial resistance has now become a worldwide issue as a consequence of the haphazard use of antimicrobials. Place and duration: This study was done in the Department of Microbiology, Novus Path Labs, India and Nepal and its associates Hospitals. Pseudomonas sp. was isolated from different clinical samples like sputum, pus. Identification of Pseudomonas sp. was done by biochemical tests. Antibiotic susceptibility test (AST) was done by Kirby-Bauer disc diffusion method. Data Analysis was done by percentage method. Extended-spectrum ?-lactamase (ESBLs) production was detected by the combined disc diffusion test. Sensitivity was seen in colistin and polymyxin B-100%, meropenem-86.58% and piperacillin-tazobactam- 82.92%. Resistance was seen in co-trimoxazole-80.48%, cefixime-82.92%, cefoperazone/sulbactam-59.75% and ciprofloxacin- 39.02%.