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Chromatography is defined as a set of techniques which is used for the separation of constituents in a mixture. This technique involves 2 phases stationary and mobile phases. The separation of constituents is based on the difference between partition coefficients of the two phases. The chromatography term is derived from the greek words namely chroma (colour) and graphein (to write). The chromatography is very popular technique and it is mostly used analytically. There are different types of chromatographic techniques namely Paper Chromatography, Gas Chromatography, Liquid Chromatography, Thin Layer Chromatography (TLC), Ion exchange Chromatography and lastly High Performance Liquid Chromatography (HPLC) HPLC is the dominant separation technique to detect, separate and quantify the drug. A number of chromatographic parameters were analyzed to optimize the method like sample pre treatment, choosing mobile phase, column, detector selection. The objective of this article is to review the method development, optimization and validation. HPLC method development depends on chemical structure of the molecules, synthetic route, solubility, polarity, pH and pKa values, and functional groups activity etc. Validation of HPLC method as per ICH Guidelines gives information regarding various stages and knowing characteristics like Accuracy, specificity, linearity limit of detection, limit of quantification.