Published Paper Details:

Dental caries is AN irreversible microbic sickness of the calcified tissues of the teeth, characterized by demineralization of the inorganic portion and destruction of the organic substance of the tooth, which frequently results in cavitation. Understanding the polymicrobial ecology in pulp-dentin complicated & periapical tissues is very important for the reduction and elimination of microorganism infection. The present study aims to spot the predominant aerobic and anaerobic microorganisms in varied stages of a dentinal cavity, pulpal and periapical lesions, thus providing a principle for sickness bar and higher treatment set-up AIMS AND OBJECTIVES Quantitative analysis of predominant microbial species in Enamel caries, Dentinal caries, and in pulpal infections without periapical lesion and with periapical lesion. METHODOLOGY Collection of samples categorized Into 4 groups to identify the predominant aerobic and anaerobic microorganisms in various stages of dentinal caries, pulpal and periapical lesions In each group 2 samples will be collected for aerobic and anaerobic culture. Under strict isolation with rubber dam, Dentinal caries and Enamel caries from the subjects teeth will be excavated using sterilised sharp spoon excavator . Superficial enamel and dentinal caries has to be removed before entering pulp chamber using high speed airotor hand piece. Under strict isolation with rubber damsamples from the subjects teeth will be excavated using sterile sharp spoon excavator and root canal instruments from the pulp chamber and root canal .Samples were collected in transporting medium and sent to laboratory CONCLUSION The study shows the quantity and prevalence of anaerobic and aerobic bacterias in each satge of dental caries.