Published Paper Details:

During extensive survey of viral disease on medicinal plants of family Euphobriaceae in the summer season of 2006-07 at different districts of Eastern Uttar Pradesh (Gorakhpur, Deoria ,Kushinagar, Maharajganj), a variety of symptoms were recorded on Phyllanthus niruri plants. The infected plants showed mosaic and mottling. Yellow-green mosaic symptoms followed by yellow patches intermingled with normal green some time whole plant leaves become white and in advanced stage of infection defoliation take place. At several locations of survey areas on P. niruri where manifested with whitefly (Bemisia tabaci) were recorded. Infected plants samples were examined by electron microscopy and nested PCR by using CRv301 and CRc1152 primer pairs. The electron micrographs prepared with leaf dip method revealed the presence of geminate particles of size 20 nm in diameter. PCR products of the expected size ~870bp, were obtained from P. niruri samples. The results of the PCR investigation revealed that the Phyllanthus niruri plant showing yellow green mosaic and complete white leaves was positive for Ageratum yellow vein virus. The virus isolate was identified as Ageratum yellow vein virus of genus Begomovirus and family Geminiviridae. This is the first report of occurrence of Ageratum yellow vein virus on P. niruri from India on the basis of symptomatology, particle morphology and PCR analysis.