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Expressing gratitude or thankfulness is not only a 'feel good' emotion attached with the organizational environment but can help employees in many ways. Practising gratitude generates a sense of worth in an employee and also has physical, psychological and interpersonal benefits. Job satisfaction on the other hand is the extent of contentment, satisfaction and self-motivation to do the current job The sample consisted of 100 employees of which 50 were males and 50 were females between the age group 35-45 years from aviation sector. For the purpose of assessment Gratitude Questionnaire (GQ-6) by McCullough (2002) and Minnesota satisfaction questionnaire (short-form) by David J. Weiss, Rene V. Dawis, George W. England and Lloyd H. Lofquist (1977) were used.The results consist of Descriptive Statistics (i.e., mean and standard deviation), Pearson Correlation and Independent t-Test Analysis. Findings suggest that the average level of gratitude and job satisfaction of males and females are same. Further, positive correlation between gratitude and job satisfaction was established which was significant at the 0.01 level with a correlation of 0.695. On analysing the t-test, no significant difference between gratitude and job satisfaction of males and females was found. Thus, the relationship between gratitude and job satisfaction in aviation sector among males and females is positive but no significant difference between males and females.