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Background: - Bacterial vaginosis (BV) is the most common cause of vaginal discharge among women in reproductive age. BV is associated with alteration of normal vaginal flora. BV is a sexually associated condition. It is characterized by increase in vaginal pH and presence of vaginal lactobacilli, Gardnerella vaginalis and Mobiluncus. BV is of special public health concern in India because of the high burden of reproductive and pregnancy related morbidity. High co-infection rates with sexually transmitted infections (STI) can increase the possibility of BV. BV can also increase the risk of pelvic inflammatory disease (PID). Pregnant female with BV can also increase the risk of preterm labour, premature rupture of membranes, chorioamnionitis and postpartum endometritis. Methods: - A total of 250 samples of vaginal fluid samples were coming to departmental laboratory of Department of microbiology of the patients attending OPD Gynaecology and Obstetrics department of Santosh Medical College & Hospital, Ghaziabad in one year. High vaginal swab samples were taken from females were whiff test, gram stained, BV Blue test & RT-PCR were done. Diagnosed on the basis of Amsel�s criteria, Nugent scoring, BV Blue test & RT-PCR for bacterial vaginosis. Results: - Prevalence of BV were 66.8%. According to various tests it was found that Amsel�s criteria shows 74%, Nugent scoring showed 66.8%, BV Blue test showed 68.8% & RT-PCR showed 66.8% bacterial vaginosis positive. Conclusion: - Bacterial vaginosis can be considered a health problem among females. As Nugent scoring was considered as the gold standard method for diagnosis of bacterial vaginosis. As concluded that RT-PCR test, Amsel�s criteria and Nugent scoring test are most sensitive and specific.