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Derivative UV spectrophotometry is an analytical technique used in multi component analysis. It has enormous implication commonly in obtaining mutually qualitative and quantitative in order from spectra that are of unresolved bands, with respect to qualitative and quantitative analysis, it uses first or higher derivatives of absorbance in accordance with wavelength. [1] Derivative spectroscopy is the simplest method for an increasing selectivity is derivatization of spectra. This method is used when the sample of drug shows large irrelevant absorption. It involves conversion of normal spectrum to its first, second and higher derivative spectra where its amplitude in the derivative spectrum is proportional to the concentration of the analyte provided that Beer�s law is obeyed by the fundamental spectrum. This article provides you a clear view of derivative spectroscopy and its advantages and disadvantages in analysis of the sample.