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For the development of aquaculture, a good knowledge of the mechanisms regulating the reproductive function in fish is required as such knowledge makes it possible to exercise varying degrees of control on the different stages of reproduction to adapt the techniques to fit fish culturist. Though, reproductive physiology has progressed considerably in recent years owing to the development of new & varied techniques (Billiard & Marcel, 1986; Sinha & Srivastava, 1991; Shankar Das and Kulkarni, 2007) but it needs more studies on the biochemical aspects especially the role of blood in fresh water fish. Thus, it appears that fluctuations in blood constituents especially the inorganic ions of the Indian fresh water fish with relation to their reproductive cycle have not been studied in detail, while among the physiological parameters; reproduction is one of the most important phenomena of an animal to continue its existence. It is also known that vitamins play some important role in the development of germ cells as both Vit �B� & �C� are concerned with maturation of oocytes. It has also been concluded that the yolk precursor (vitellogenin) are synthesized in liver & brought to the oocytes by blood (Bohemen et.al; 1981). As blood performs the vital function of nutrition & respiration to each and every tissue of the body, its (blood) constituents must take active role in such processes. Therefore, any variations in these constituents of blood even due to season would have some effect on the Gonadal cycle as well, but only a few work is available on some of these physiological parameters (Idler & Bitner 1958; Gill et. al., 1983; Singh, 1990, 1996; Panday et.al., 1993) and thus needs further studies. In this paper we determine Cholesterol, Lactic Acid, Pyruvic Acid, Ascorbic Acid, and Biochemical Parameters in Serum of the Clarias Batrachus Fish.