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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
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Published Paper Details:
Paper Title
ANALYTICAL METHOD VALIDATION OF VITAMIN A ACETATE IN SUNFLOWER BY LIQUID CHROMATOGRAPHY METHOD (RP- HPLC)
Authors
Chavanke Priyanka
Keywords
Vitamine A Acetate
Abstract
A simple, efficient and reproducible RP-HPLC method for the determination of
Vitamin A acetate in sunflower oil has been developed and validated. The
Chromatographic Separation was carried out on C18 Column (inertsil ODS)
column using the mobile phase consists of Methanol: Acetonitrile (70:30). The
mobile phase was flowed at the rate of 1.0 ml/min and effluent was detected at
325 nm. The retention times of Vitamin A acetate was 2.928 min. The method
was validated according to ICH guidelines and the acceptance criteria for system
suitability, specificity, linearity, precision, robustness and ruggedness were met in
all cases. The method was linear in the range of 0.05-10 ppm of the operating
concentration of Vitamin A acetate (r2 = 0.998)). The percentage relative standard
deviation for Intermediate precision was found to be less than 2.0%. Hence, the
method could be successfully applied for routine analysis of Vitamin A acetate
from Sunflower oil.
IJCRT's Publication Details
Unique Identification Number - IJCRT2010350
Paper ID - 199912
Page Number(s) - 2571-2580
Pubished in - Volume 8 | Issue 10 | October 2020
DOI (Digital Object Identifier) -
Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
Chavanke Priyanka, "ANALYTICAL METHOD VALIDATION OF VITAMIN A ACETATE IN SUNFLOWER BY LIQUID CHROMATOGRAPHY METHOD (RP- HPLC)", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.8, Issue 10, pp.2571-2580, October 2020, Available at :http://www.ijcrt.org/papers/IJCRT2010350.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
ISSN and 7.97 Impact Factor Details
ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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