Published Paper Details:

Restriction digestion of pUC18 and pBR322 will be done using restriction enzymes such as BamH I and EcoR I respectively. The Hybrid plasmid (pUC18 + pBR322) will be constructed by adding T4 DNA ligase to the digested vectors and transformed to the competent cells of DH5? E.coli strain with the help of Calcium chloride. Blue- white Screening would be done for the transformed cells where the white colonies would be selected and the possible results might be of transformed cells containing either pBR322 alone or the hybrid plasmid. The selected white colonies will be subjected to antibiotic screening by adding ampicillin. The colonies that will be formed after the addition of ampicillin are supposed to be considered as the cells having hybrid plasmids. Further, the transformed DH5? cells containing the hybrid plasmid would be subjected to stability studies such as determination of growth rate by comparing the growth rates of control DH5? cells, transformed DH5? cells containing pUC18 and pBR322 individually with the transformed DH5? cells containing the hybrid plasmid. Other stability studies such as thermal resistance of transformed DH5? cells containing the hybrid plasmid would be determined. From the above studies, it would be possible to create a novel DH5? cell strain with added advantages of increased growth rate and thermal resistance.