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  Published Paper Details:

  Paper Title

BIOANALYTICAL METHOD DEVELOPEMENT AND VALIDATION OF POSACONAZOLE IN HUMAN PLASMA BYRP-HPLC METHOD

  Authors

  Dr. Sonali P. Mahaparale,  Arti Muneshwar Yadav

  Keywords

Posocanazole, Reverse phases HPLC, Accuracy, Precision,

  Abstract


A simple, rapid, selective, sensitive, accurate and precise High Performance Liquid Chromatography (HPLC) with UV detection method has been developed and validated for determination of Posaconazole in Human plasma. C18 (250x4.6mm) column was used with the mobile phase containing a mixture of Acetonitrile: Water (55:45v/v). The flow rate was 0.8ml/min and drug was monitored at 262nm. In analytical method development linearity for Posaconazole was found to be 5-100�g/ml with regression coefficient 0.9954. The accuracy and recovery was found within limit. The robustness study was performed and the method was found to be fully robust. Plasma samples were processed using acetonitrile as precipitating agent to extract drug. The linearity for Posaconazole was found to be 0.15 to 50?g/ml with regression coefficient (r2) 0.9955. The recovery was found to be 85.63%. Stability studies for freeze thaw cycle, short term stability and long term stability was done. The % CV for stability study was found within a limit.

  IJCRT's Publication Details

  Unique Identification Number - IJCRT2006546

  Paper ID - 196189

  Page Number(s) - 4000-4009

  Pubished in - Volume 8 | Issue 6 | June 2020

  DOI (Digital Object Identifier) -   

  Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882

  E-ISSN Number - 2320-2882

  Cite this article

  Dr. Sonali P. Mahaparale,  Arti Muneshwar Yadav,   "BIOANALYTICAL METHOD DEVELOPEMENT AND VALIDATION OF POSACONAZOLE IN HUMAN PLASMA BYRP-HPLC METHOD", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.8, Issue 6, pp.4000-4009, June 2020, Available at :http://www.ijcrt.org/papers/IJCRT2006546.pdf

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ISSN: 2320-2882
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Journal Starting Year (ESTD) : 2013
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ISSN and 7.97 Impact Factor Details


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ISSN: 2320-2882
Impact Factor: 7.97 and ISSN APPROVED
Journal Starting Year (ESTD) : 2013
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