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  Published Paper Details:

  Paper Title

F2 SCREENING OF SESAME (SESAMUM INDICUM L.) GENOTYPES BY USING MOLECULAR MARKERS

  Authors

  Swathi R S,  Dr. Bharose A A,  Nalini Arun Shinde

  Keywords

Seed coat colour, bulk segregant analysis, RAPD, SSR, polymorphism

  Abstract


Bulk segregant analysis an approach to analyzing the F2 individuals of white seeded IC-203871 X brown seeded AKT-101 to screen and identify the markers linked to the seed coat colour trait in sesame. 2 DNA bulks viz., white and brown bulks was setup by pooling equal amount of DNA from 10 randomly selected plants generated from a segregating population from a single cross. A total of 35 (15 RAPD; 20 SSR) primers were screened out of which 2 primers were polymorphic between parents and F2 bulks were then used to screen 60 segregating F2 progeny. RPI-7 and Hs-1871 showed linkage with the brown seed coat colour in sesame. Out of 60 individuals of the progeny, the polymorphic band was seen in 26 (RAPD) and 14 (SSR) samples showing it linkage with the brown seed coat colour. The inheritance for seed coat colour genes was segregated in the ratio 9:3:4.

  IJCRT's Publication Details

  Unique Identification Number - IJCRT2006413

  Paper ID - 196017

  Page Number(s) - 2999-3005

  Pubished in - Volume 8 | Issue 6 | June 2020

  DOI (Digital Object Identifier) -   

  Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882

  E-ISSN Number - 2320-2882

  Cite this article

  Swathi R S,  Dr. Bharose A A,  Nalini Arun Shinde,   "F2 SCREENING OF SESAME (SESAMUM INDICUM L.) GENOTYPES BY USING MOLECULAR MARKERS", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.8, Issue 6, pp.2999-3005, June 2020, Available at :http://www.ijcrt.org/papers/IJCRT2006413.pdf

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ISSN: 2320-2882
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ISSN and 7.97 Impact Factor Details


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ISSN: 2320-2882
Impact Factor: 7.97 and ISSN APPROVED
Journal Starting Year (ESTD) : 2013
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