Published Paper Details:

ABSTRACT In-vitro studies were carried out on Aspergillus niger, an indigenous strain, to determine the most optimize conditions for high xylanase production. The selected strain was boosted for xylanase enzyme production in czapek�s dox broth medium using different carbon and nitrogen sources. Maximum enzyme activity was detected when xylan was used as carbon source and yeast extract was used as nitrogen source. Shake culture conditions, 1.0% NaCl, higher inoculums dose and 06 days of incubation were observed with high xylanase production. Submerged condition was found superior over solid-state condition; however, wheat bran substrate yielded more xylanase over others. Optimum pH and temperature for xylanase activity were found to be 7.0 and 28oC. The enzyme was produced and extracted under optimized conditions and compared with values of before optimization. It was then purified and characterized for stability at different temperatures and pH. A high amount of xylanase was formed under optimized conditions and was found stable up to 45oC temperature and at pH 8.0.