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ABSTRACT:- In the present article attempt has been made to explain the basic ideas of different separation techniques (Chromatography). A sophisticated instrument, chromatography involve various type of chromatography divisions which allow to separate the crude drug or mixture of components. In this technique two phase are use mobile phase and stationary phase, in which one phase may be polar and another phase are non-polar according to the nature of sample. They focus on bio-analytical techniques which could help them in separating the most active elements from the crude mixtures. It also includes various different type of quantitative and qualitative techniques to estimation of biological and pharmaceutical samples. It is most versatile, safest, dependable and fastest techniques for qualitative control of drug components. While basic chromatography still prevails as the most important analytical tools in molecular chemistry. This article focuses on the various types of chromatography and it�s principle, working and applications.