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The world is now dealing with various environmental problems where nanobiotechnology is emerging as a new avenue for safer and cleaner environment. The studies on CO2 sequestration are being pursued all across the world. Many research works are focused on the development of single enzyme nanoparticles of enzymes were targeted for environmental bioremediation. This work is used to improve the stability and the active life of biocatalyst by using the approach of stabilization and immobilization, either singly or in combination. There are researches on the development of synthesis of single enzyme nanoparticles of carbonic anhydrase (SEN-CA). Individual enzyme molecules are stabilized within a biopolymeric silica network of nanometer scale thickness. A key result is that stabilization of the enzyme activity was achieved with minimal substrate mass transfer limitation compared to enzyme entrapped in larger scale materials. The development of stabilized SEN as soluble individual SEN particles also provides the opportunity to further immobilized on to the different matrices. These SEN provides major advantages such as stabilization of the activity, reduced mass-transfer limitation relative to large-scale immobilizations, and process ability into additional forms including hierarchical architectures. There are reports on biomimetic approach for sequestration of CO2into calcium carbonate using stabilized/immobilized CA as a non-site scrubber. Carbon sequestration by biological means will produce safe, environmental friendly products like calcium carbonate. It will be a permanent method for disposing CO2which shall make our environment cleaner, greener and sustainable.