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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
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Paper Title
PARTIAL PURIFICATION AND CHARACTERIZATION OF ALPHA AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS BY SOLID STATE FERMENTATION
Authors
REKHANI JYOTI PAL
Keywords
alpha amylase, agro products, Bacillus amyloliquefaciens
Abstract
Among different types of enzymes obtained from microbial sources, amylases are the most widely used in industries. In the present study, different agro products were used for production of alpha amylase by using Bacillus amyloliquefaciens. The present study showed that wheat flour and wheat bran has higher efficiency in ?- amylase production using Bacillus amyloliquefaciens. The partial purification of the enzyme was done by fractionation of raw extract with ammonium sulphate salt in a variety of saturated degree to get the partial purified enzyme. The partial characterization of partially purified enzyme was done by determination of the optimum pH and temperature at which the enzyme activity is maximum. The purified alpha amylase obtained from Bacillus amyloliquefaciens make it good candidate for wide application as additives and starch modification.
IJCRT's Publication Details
Unique Identification Number - IJCRT1705425
Paper ID - 181453
Page Number(s) - 1289-1301
Pubished in - Volume 6 | Issue 1 | January 2018
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Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
REKHANI JYOTI PAL, "PARTIAL PURIFICATION AND CHARACTERIZATION OF ALPHA AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS BY SOLID STATE FERMENTATION", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.6, Issue 1, pp.1289-1301, January 2018, Available at :http://www.ijcrt.org/papers/IJCRT1705425.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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