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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
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Paper Title
FACTORS AFFECTING MICROPROPAGATION OF ANOGEISSUS ACUMINATE. A TREE OF STRESSED ECOSYSTEM.
Authors
Narpat Singh Deora
Keywords
: explants , in vitro, medium ,callus. Shoot induction, seedling
Abstract
Various Experiments viz. Types of Seedling Explants (epicotyledonary nodal segments, cotyledonary nodal segments, hypocotyl, cotyledon etc.) types of growth regulators (KN, BAP,IAA,NAA) and its various concentration were conducted to standardized the methods of in vitro micropropagation of A. acuminata. Out of Various types of explants used, the cotyledonary nodal shoot segments found the best for shoot induction. Highest number of shoot bud proliferation i.e. 15-20 shoots was found on MS medium supplemented with IAA 0.1mg/l + BAP 1.5 mg/l, while only 4-6 shoots were produced from epicotyledonary nodal shoot segments .Poor response i.e. only callusing was observed on hypocotyl and cotyledon explants. On higher concentration of BAP i.e. more than 2.5 mg/l, although number of shoot were increased but they remain dwarf. On Higher concentration of IAA and NAA, callusing was observed.
IJCRT's Publication Details
Unique Identification Number - IJCRT1135055
Paper ID - 235619
Page Number(s) - 376-379
Pubished in - Volume 6 | Issue 1 | February 2018
DOI (Digital Object Identifier) -
Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
Narpat Singh Deora, "FACTORS AFFECTING MICROPROPAGATION OF ANOGEISSUS ACUMINATE. A TREE OF STRESSED ECOSYSTEM.", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.6, Issue 1, pp.376-379, February 2018, Available at :http://www.ijcrt.org/papers/IJCRT1135055.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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