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The anti-microbial activity of the formulated herbal hand wash gel was tested against Escherichia coli, Staphylococcus aureus and Salmonella by spread plate techniques and the results obtained were compared with commercial antibacterial standards. Alsothe efficiency was checked by using the hand wash gel on volunteers. The results from the present work suggest and support the incorporation and utilization of herbs in theformulations to give better effect. Herbal medicines are significant part of healthcare throughout the world. Herbal medicines have been extensively utilized as effectual remedies for the prevention and management of multiple health conditions. Hands are a prime mode of transmission of microbes and nosocomial infections. Handwashing is extremely imperative in healthcare and domestic sector. Numerous of the antiseptic hand wash available in the market are alcohol based sanitizers which have some adverse effects. To avoid these adverse effects like itching, drying, irritation, dermatitis etc., of the synthetic handwash formulations an attempt has been made to formulate a herbal hand wash using extracts of Eucalyptus globulus extracts. The results from the present work support the incorporation and utilization of herbs in the formulations to give a better effect. Herbal hand wash evaluated by tested parameters like physical parameters like colour, fragrance and chemical parameters like pH, Viscosity, Foam height, Foam retention, Skin irritation test etc. and obtained results were in the acceptable limits with less or